Journal: Breast Cancer (Tokyo, Japan)

Article Title: IRSN-23 gene diagnosis enhances breast cancer subtype classification and predicts response to neoadjuvant chemotherapy: new validation analyses

doi: 10.1007/s12282-025-01687-6

Figure Lengend Snippet: Overview of IRSN-23 pCR-prediction without anti-HER2 therapy in breast cancer. A Meta-analysis of all datasets. The gray-shaded areas show the results of the present study. B Pooled analysis of each subtype and treatment. C IRSN-23 immune scores (IS) and pCR rates for each subtype. The solid lines and bands represent loess regression fitting (span = 2), and 95% confidence intervals were implemented using the R function geom_smooth. Abbreviations in the figure are as follows: GPL570 Affymetrix Human Genome U133 Plus 2.0 Array, GPL571 Affymetrix Human Genome U133A 2.0 Array, GPL96 Affymetrix Human Genome U133A Array, GPL6884 Illumina HumanWG-6 v3.0 expression beadchip, GPL1352 Affymetrix Human X3P, GPL6480 Agilent-014850 Whole Human Genome Microarray 4 × 44 K G4112F, A anthracycline, T taxane, Pac paclitaxel, Doc docetaxel, Ixa ixabepilone. Predictors of chemotherapy sensitivity and clinical utility

Article Snippet: Gene expression analysis was performed using Affymetrix U133A Plus 2.0 DNA microarray since the last publication.

Techniques: Expressing, Microarray

Journal: Journal of Allergy

Article Title: How Can Microarrays Unlock Asthma?

doi: 10.1155/2012/241314

Figure Lengend Snippet: Overview of the production and use of expression microarrays. 3′ Expression arrays use synthetically derived oligo probes with design based on mRNA Databases (RefSeq mRNAs, GenBank mRNAs, and ESTs from dbEST) or cDNA derived from bacterial libraries (see ). Sample mRNA can be labeled using two methods (a) Cy3/Cy5 labeling: sample mRNA is reverse transcribed into cDNA and Cy3 is added to one sample and Cy5 to another. Both labeled samples are hybridized to the same microarray. (b) 3′ IVT array: sample mRNA is reverse transcribed to cDNA using oligo(dT) primers, to provide a template for transcription. Using biotin-conjugated nucleotides, the template cDNA is then converted to amplified RNA (aRNA). The biotin-labeled aRNA samples are then fragmented and hybridized onto 3′ expression arrays. A biotin binding fluorescent stain is added to the microarray after hybridization. (c) Affymetrix HuExon 1.0 ST: sample mRNA is reverse transcribed to cDNA using random primers, to provide a template for transcription. The resulting RNA is then reverse transcribed in the presence of dUTPs which are incorporated occasionally into the cDNA sequence instead of dTTP. An enzyme is then used to cleave the cDNA at the site of dUTP incorporation and fragments are terminally labeled before hybridization onto the array. The microarray is then washed and stained after hybridization.

Article Snippet: 2010 , Transglutaminase 2, a novel regulator of eicosanoid production in asthma revealed by genomewide expression profiling of distinct asthma phenotypes , Affymetrix Human U133A DNA microarrays Human Genome U133 Plus 2.0 GeneChip arrays , GSE13785 , [ ] .

Techniques: Expressing, Derivative Assay, Labeling, Microarray, Amplification, Binding Assay, Staining, Hybridization, Sequencing

Journal: Journal of Allergy

Article Title: How Can Microarrays Unlock Asthma?

doi: 10.1155/2012/241314

Figure Lengend Snippet: The GEO accession number for microarray studies conducted on asthma.

Article Snippet: 2010 , Transglutaminase 2, a novel regulator of eicosanoid production in asthma revealed by genomewide expression profiling of distinct asthma phenotypes , Affymetrix Human U133A DNA microarrays Human Genome U133 Plus 2.0 GeneChip arrays , GSE13785 , [ ] .

Techniques: Microarray, Expressing, Clone Assay, Infection, Functional Assay, Sequencing, Synthesized